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Western blot analysis in MDA-MB231 cell line lysates (35ug/lane).
Western blot analysis in CHO cell line lysates (35ug/lane).
Confocal immunofluorescent analysis of SORBS1 Antibody with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Western blot analysis in MDA-MB231 cell line lysates (35ug/lane).

SORBS1 Antibody

CATALOG NUMBER: 55-343

Clonality:
Polyclonal
Tested Applications:
IF, WB
Host Species:
Rabbit
Species Reactivity:
Hamster, Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Hamster, Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This SORBS1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 866-892 amino acids from the Central region of human SORBS1.
Conjugate:
Unconjugated
Tested Applications:
IF, WB
Application Note:
For WB starting dilution is: 1:1000
For IF starting dilution is: 1:10~50
Predicted Molecular Weight:
143 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SORBS1
Additional Names:
Sorbin and SH3 domain-containing protein 1, Ponsin, SH3 domain protein 5, SH3P12, c-Cbl-associated protein, CAP, SORBS1 (HGNC:14565)
Protein Accession Number:
Q9BX66
Protein Gi Number:
317373504
Ncbi Gene Id Number:
10580
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Plays a role in tyrosine phosphorylation of CBL by linking CBL to the insulin receptor. Required for insulin-stimulated glucose transport. Involved in formation of actin stress fibers and focal adhesions (By similarity).
Background Reference 1:
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010)Joslyn, G., et al. Alcohol. Clin. Exp. Res. 34(5):800-812(2010) Gehmlich, K., et al. Eur. J. Cell Biol. 89(5):351-364(2010) Yamada, Y., et al. Hypertens. Res. 32(5):325-331(2009) Fernow, I., et al. BMC Cell Biol. 10, 80 (2009) :

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-343

List Size:
400 ul

List Price:

$495.00

Datasheet
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