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43-383 (0.1ug/ml) staining of Human Cerebellum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
43-383 (0.1ug/ml) staining of Mouse Brain lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
43-383 (0.1ug/ml) staining of Human Cerebellum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

SOX10 Antibody

CATALOG NUMBER: 43-383

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse
Homology:
Expected Species Reactivity based on sequence homology: Rat, Dog, Cow, Pig
Immunogen:
The immunogen for this antibody is: C-DAKAQVKTETAGPQ
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 50kDa band observed in Human Brain (Cerebellum) and approx. 52kDa in Mouse Brain lysates (calculated MW of 49.9kDa according to Human NP_008872.1 and Mouse NP_035567.1). Recommended concentration: 0.03-0.1ug/ml. Primary incubation 1 hour at room temperature.
Predicted Molecular Weight:
Approx 48kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
SOX10
Additional Names:
SOX10, SRY (sex determining region Y)-box 10 , DOM, MGC15649, WS4, OTTHUMP00000028515, SRY-related HMG-box gene 10, dominant megacolon, mouse, human homolog of
Protein Accession Number:
NP_008872.1
Protein Gi Number:
5902104
Ncbi Gene Id Number:
6663
Background Reference 1:
Ghislain J, Charnay P.Control of myelination in Schwann cells: a Krox20 cis-regulatory element integrates Oct6, Brn2 and Sox10 activities.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-383

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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