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Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using SOX10 antibody (clone SBX10-1). Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
Western blot testing of 1) partial recombinant protein and 2) A375 cell lysate using SOX10 antibody. Predicted molecular weight ~50 kDa.
IHC testing of FFPE melanoma with SOX10 antibody (clone SBX10-1). Required HIER: boil sections in pH6 10mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using SOX10 antibody (clone SBX10-1). Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SOX10 Antibody [SBX10-1]

CATALOG NUMBER: 33-852

Clonality:
Monoclonal
Tested Applications:
IF, IHC-P, WB
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Immunogen:
Amino acids 115-269 of the human protein were used as the immunogen for the SOX10 antibody.
Conjugate:
Unconjugated
Tested Applications:
IF, IHC-P, WB
Application Note:
Immunofluorescence: 1-2 ug/ml
Western blot: 1-2 ug/ml
Immunohistochemistry (FFPE): 1-2 ug/ml for 30 min at RT
Prediluted IHC only format: incubate for 30 min at RT (1)
Titering of the SOX10 antibody may be required for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Purification:
Protein G affinity chromatography
Clonality:
Monoclonal
Isotype:
IgG2b, kappa
physical-state:
Liquid
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Storage Conditions:
Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
Ncbi Official Symbol:
SOX10
Additional Names:
SOX10 Antibody: SRY (sex determining region Y)-box 10, DOM,WS4, PCWH, WS2E, WS4C
Ncbi Gene Id Number:
6663
User Note:
Optimal dilutions for each application to be determined by the researcher
Background:
SOX10 is a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. It may act as a transcriptional activator after forming a protein complex with other proteins. This protein acts as a nucleocytoplasmic shuttle protein and is important for neural crest and peripheral nervous system development. [RefSeq]

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

33-852

List Size:
100 ug

List Price:

$626.00

Datasheet
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