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Western blot analysis of SOX2 in 3T3 cell lysate with SOX2 antibody at (A) 1 and (B) 2 μg/mL.
Immunocytochemistry of SOX2 in 3T3 cells with SOX2 antibody at 5 μg/mL.
Immunofluorescence of SOX2 in 3T3 cells with SOX2 antibody at 20 μg/mL.
Immunohistochemistry of SOX2 in mouse brain tissue with SOX2 Antibody at 5 μg/mL.
Immunofluorescence of SOX2 in mouse brain tissue with SOX2 Antibody at 20 μg/mL.<br><br>Green: SOX2 antibody (5583) <br> Red: Phylloidin staining<br> Blue: DAPI staining
Western blot analysis of SOX2 in 3T3 cell lysate with SOX2 antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

SOX2 Antibody

CATALOG NUMBER: 5583

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Sheep: (100%), Chicken: (100%), Horse: (83%), Pig: (100%)
Immunogen:
SOX2 antibody was raised against a 15 amino acid synthetic peptide near the amino terminus of human SOX.
The immunogen is located within the first 50 amino acids of SOX2.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, IHC-P, WB
Application Note:
SOX2 antibody can be used for detection of SOX2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL and immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples; Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1282 - 3T3 (NIH) Cell Lysate
Positive Control 2:
Cat. No. 17-201 - 3T3/BALB Cell Slide
Purification:
SOX2 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
SOX2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
SOX2 antibody can be stored at 4°C, stable for up to one year. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SOX2
Additional Names:
SOX2 Antibody: ANOP3, MCOPS3, Transcription factor SOX-2
Protein Accession Number:
NP_003097
Protein Gi Number:
28195386
Ncbi Gene Id Number:
6657
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
SOX2 Antibody: SOX2 is a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. SOX2 is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. The role of SOX2 in embryonic development suggested that it might be useful in the creation of stem cells that might be useful in cell replacement therapies in the treatment of degenerative diseases. Artificial stem cells, termed induced pluripotent stem (iPS) cells, can be created by expressing SOX2 and the transcription factors POU5F1, Klf4 and Lin28 along with c-Myc in mouse fibroblasts. Other experiments have shown that iPS cells could be generated using expression plasmids expressing POU5F1, SOX2, KlfF4 and c-Myc, eliminating the need for virus introduction.
Background Reference 1:
Hever AM, Williamson KA, and van Heyningen V. Developmental malformations of the eye: the role of PAX6, SOX2 and OTX2. Clin. Genet.2006; 69:459-70.
Background Reference 2:
Carpenter MK, Rosler E, and Rao MS. Characterization and differentiation of human embryonic stem cells. Cloning Stem Cells2003; 5:79-88.
Background Reference 3:
Takahashi K and Yamanaka S. Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell2006; 1263:663-76.
Background Reference 4:
Okita K, Nakagawa M, Hyenjong H, et al. Generation of mouse induced pluripotent stem cells without viral vectors. Science2008; 322:949-53.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5583

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

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