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Western blot analysis of extracts of various cell lines, using SOX5 antibody (22-607) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 3s.
Immunohistochemistry of paraffin-embedded rat kidney using SOX5 antibody (22-607) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using SOX5 antibody (22-607) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human kidney cancer using SOX5 antibody (22-607) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using SOX5 antibody (22-607) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using SOX5 antibody (22-607) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of MCF7 cells using SOX5 antibody (22-607).
Western blot analysis of extracts of various cell lines, using SOX5 antibody (22-607) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 3s.

SOX5 Antibody

CATALOG NUMBER: 22-607

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human SOX5 (NP_694534.1).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:100
Positive Control 1:
HepG2
Positive Control 2:
SH-SY5Y
Positive Control 3:
U-87MG
Positive Control 4:
Mouse heart
Predicted Molecular Weight:
Observed: 70kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
SOX5
Additional Names:
SOX5, L-SOX5, L-SOX5B, L-SOX5F, RP11-444D3.1
Ncbi Gene Id Number:
6660
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional regulator after forming a protein complex with other proteins. The encoded protein may play a role in chondrogenesis. A pseudogene of this gene is located on chromosome 8. Multiple transcript variants encoding distinct isoforms have been identified for this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

22-607

List Size:
100 uL

List Price:

$436.00

Datasheet
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