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Antibody used in WB on Human HeLa at 0.2-1 ug/ml.
Antibody used in WB on Human HepG2 at 2.0 ug/ml (Lane A: Primary Antibody and Lane B: Primary Antibody + Blocking Peptide ).
Antibody used in WB on HepG2 at 2.0 ug/ml (Lane A: Primary Antibody and Lane B: Primary Antibody + Blocking Peptide ).
Antibody used in WB on Human HeLa at 0.2-1 ug/ml.

SOX5 Antibody

CATALOG NUMBER: 27-828

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Dog, Human, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Dog, Human, Rat
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SOX5.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
SOX5 antibody can be used for detection of SOX5 by ELISA at 1:62500. SOX5 antibody can be used for detection of SOX5 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1211 - HepG2 Cell Lysate
Predicted Molecular Weight:
42 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store SOX5 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
SOX5
Additional Names:
SOX5, L-SOX5, L-SOX5B, L-SOX5F, RP11-444D3.1
Protein Accession Number:
NP_821078
Protein Gi Number:
30061558
Ncbi Gene Id Number:
6660
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
SOX5 encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional regulator after forming a protein complex with other proteins. The encoded protein may play a role in chondrogenesis. A pseudogene of this gene is located on chromosome 8.
Background Reference 1:
Ikeda, T., et al., () Gene 298 (1), 59-68 2002.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

27-828

List Size:
100 ul

List Price:

$519.00

Datasheet
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