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Antibody used in WB on Human HepG2 cells at 2.5 ug/ml.
Antibody used in WB on Human HepG2 cells at 2.5 ug/ml.

SOX9 Antibody

CATALOG NUMBER: 31-182

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Dog, Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Dog, Human, Mouse, Rat
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SOX9.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
SOX9 antibody can be used for detection of SOX9 by ELISA at 1:12500. SOX9 antibody can be used for detection of SOX9 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1211 - HepG2 Cell Lysate
Predicted Molecular Weight:
56 kDa
Purification:
Antibody is purified by protein A chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store SOX9 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
SOX9
Additional Names:
SOX9, CMD1, SRA1, CMPD1
Protein Accession Number:
NP_000337
Protein Gi Number:
4557853
Ncbi Gene Id Number:
6662
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
SOX9 recognizes the sequence CCTTGAG along with other members of the HMG-box class DNA-binding proteins. It acts during chondrocyte differentiation and, with steroidogenic factor 1, regulates transcription of the anti-Muellerian hormone (AMH) gene. Deficiencies lead to the skeletal malformation syndrome campomelic dysplasia, frequently with sex reversal.
Background Reference 1:
Furumatsu, T., et al., (2005) J. Biol. Chem. 280 (42), 35203-35208.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

31-182

List Size:
100 ul

List Price:

$473.00

Datasheet
Shipping Info

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