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43-573 (0.3ug/ml) staining of NIH3T3 nuclear lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
43-573 (0.3ug/ml) staining of NIH3T3 nuclear lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Sp1Antibody

CATALOG NUMBER: 43-573

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Mouse
Homology:
Expected Species Reactivity based on sequence homology: Rat, Pig
Immunogen:
The immunogen for this antibody is: C-DGIHDETAGGEGEN
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 100kDa band observed in nuclear lysates of cell line NIH3T3 (calculated MW of 80.4kDa according to NP_038700.2). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.3-1ug/ml.
Predicted Molecular Weight:
Approx 100kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
Sp1
Additional Names:
Sp1, trans-acting transcription factor 1, 1110003E12Rik, AA450830, AI845540, Sp1-1
Protein Accession Number:
NP_038700.2
Protein Gi Number:
68067398
Ncbi Gene Id Number:
20683
Background Reference 1:
Uren AG, Kool J, Matentzoglu K, de Ridder J, Mattison J, van Uitert M, Lagcher W, Sie D, Tanger E, Cox T, Reinders M, Hubbard TJ, Rogers J, Jonkers J, Wessels L, Adams DJ, van Lohuizen M, Berns A, Large-scale mutagenesis in p19(ARF)- and p53-deficient mice identifies cancer genes and their collaborative networks.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-573

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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