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Antibody used in IHC on Human Prostate at 5.0 ug/ml.
Antibody used in WB on Human Muscle at 0.2-1 ug/ml.
Antibody used in WB on Human U2OS at 1:1000.
Antibody used in IHC on Human Prostate at 5.0 ug/ml.

SP3 Antibody

CATALOG NUMBER: 25-131

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SP3.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
SP3 antibody can be used for detection of SP3 by ELISA at 1:312500. SP3 antibody can be used for detection of SP3 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. XBL-10413 - Fetal Skeletal Muscle Tissue Lysate
Predicted Molecular Weight:
82 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store SP3 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
SP3
Additional Names:
SP3, DKFZp686O1631, SPR-2, SPR2
Protein Accession Number:
NP_003102
Protein Gi Number:
38373693
Ncbi Gene Id Number:
6670
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
SP3 is a transcriptional factor that can act as an activator or repressor, probably in a isoform-specific manner. SP3 binds to GT and GC boxes promoters elements.This gene belongs to a family of Sp1 related genes that encode transcription factors that regulate transcription by binding to consensus GC- and GT-box regulatory elements in target genes. This protein contains a zinc finger DNA-binding domain and several transactivation domains, and has been reported to function as a bifunctional transcription factor that either stimulates or represses the transcription of numerous genes. Transcript variants encoding different isoforms have been described for this gene, and one has been reported to initiate translation from a non-AUG (AUA) start codon. Additional isoforms, resulting from the use of alternate downstream translation initiation sites, have also been noted.
Background Reference 1:
Whitten, C., Gene 413 (1-2), 58-66 (2008).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

25-131

List Size:
100 ul

List Price:

$519.00

Datasheet
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