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Western blot analysis in HepG2 cell line lysates (35ug/lane).
Western blot analysis in HepG2 cell line lysates (35ug/lane).

SP3 Antibody

CATALOG NUMBER: 57-717

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This SP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 292-321 amino acids from the Central region of human SP3.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
82 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SP3
Additional Names:
Transcription factor Sp3, SPR-2, SP3
Protein Accession Number:
Q02447
Protein Gi Number:
30923147
Ncbi Gene Id Number:
6670
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene belongs to a family of Sp1 related genes thatencode transcription factors that regulate transcription by bindingto consensus GC- and GT-box regulatory elements in target genes.This protein contains a zinc finger DNA-binding domain and severaltransactivation domains, and has been reported to function as abifunctional transcription factor that either stimulates orrepresses the transcription of numerous genes. Transcript variantsencoding different isoforms have been described for this gene, andone has been reported to initiate translation from a non-AUG (AUA)start codon. Additional isoforms, resulting from the use ofalternate downstream translation initiation sites, have also beennoted. A related pseudogene has been identified on chromosome 13.
Background Reference 1:
Yamaguchi, K., et al. Biochem. J. 430(1):107-117(2010)
Background Reference 2:
Jutooru, I., et al. J. Biol. Chem. 285(33):25332-25344(2010)
Background Reference 3:
Markovics, J.A., et al. J. Biol. Chem. 285(32):24695-24706(2010)
Background Reference 4:
Eckerstorfer, P., et al. Mol. Immunol. 47 (11-12), 2094-2102 (2010) :

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-717

List Size:
400 ul

List Price:

$495.00

Datasheet
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