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Antibody used in WB on Human Jurkat 0.2-1 ug/ml.
Antibody used in WB on Human Jurkat 0.2-1 ug/ml.

SP6 Antibody

CATALOG NUMBER: 30-673

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SP6.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
SP6 antibody can be used for detection of SP6 by ELISA at 1:62500. SP6 antibody can be used for detection of SP6 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1205 - Jurkat Cell Lysate
Predicted Molecular Weight:
40 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store SP6 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
SP6
Additional Names:
SP6, KLF14, MGC119662, MGC119663, MGC119664, MGC119665, EPFN, EPIPROFIN
Protein Accession Number:
NP_954871
Protein Gi Number:
42263098
Ncbi Gene Id Number:
80320
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
SP6 belongs to a family of transcription factors that contain 3 classical zinc finger DNA-binding domains consisting of a zinc atom tetrahedrally coordinated by 2 cysteines and 2 histidines (C2H2 motif). These transcription factors bind to GC-rich sequences and related GT and CACCC boxes.SP6 belongs to a family of transcription factors that contain 3 classical zinc finger DNA-binding domains consisting of a zinc atom tetrahedrally coordinated by 2 cysteines and 2 histidines (C2H2 motif). These transcription factors bind to GC-rich sequences and related GT and CACCC boxes (Scohy et al., 2000 [PubMed 11087666]).
Background Reference 1:
Nakamura, T., (2004) J. Biol. Chem. 279 (1), 626-634.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

30-673

List Size:
100 ul

List Price:

$519.00

Datasheet
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