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Western blot analysis in K562 cell line lysates (35ug/lane).
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in K562 cell line lysates (35ug/lane).

SP6 Antibody

CATALOG NUMBER: 56-797

Clonality:
Polyclonal
Tested Applications:
Flow, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This SP6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 196-225 amino acids from the Central region of human SP6.
Conjugate:
Unconjugated
Tested Applications:
Flow, WB
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
40 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SP6
Additional Names:
Transcription factor Sp6, Krueppel-like factor 14, SP6, KLF14
Protein Accession Number:
Q3SY56
Protein Gi Number:
82582258
Ncbi Gene Id Number:
80320
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
SP6 belongs to a family of transcription factors thatcontain 3 classical zinc finger DNA-binding domains consisting of azinc atom tetrahedrally coordinated by 2 cysteines and 2 histidines(C2H2 motif). These transcription factors bind to GC-rich sequencesand related GT and CACCC boxes (Scohy et al., 2000 [PubMed11087666]).
Background Reference 1:
Nakamura, T., et al. J. Biol. Chem. 279(1):626-634(2004)
Background Reference 2:
Scohy, S., et al. Genomics 70(1):93-101(2000)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-797

List Size:
400 ul

List Price:

$495.00

Datasheet
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