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Western blot analysis in Jurkat cell line lysates (35ug/lane).
SPAG1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in Jurkat cell line lysates (35ug/lane).

SPAG1 Antibody

CATALOG NUMBER: 62-012

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This SPAG1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 124-152 amino acids from the N-terminal region of human SPAG1.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
Predicted Molecular Weight:
104 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SPAG1
Additional Names:
Sperm-associated antigen 1, HSD-38, Infertility-related sperm protein Spag-1, SPAG1
Protein Accession Number:
Q07617
Protein Gi Number:
223634718
Ncbi Gene Id Number:
6674
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The correlation of anti-sperm antibodies with cases ofunexplained infertility implicates a role for these antibodies inblocking fertilization. Improved diagnosis and treatment ofimmunologic infertility, as well as identification of proteins fortargeted contraception, are dependent on the identification andcharacterization of relevant sperm antigens. The protein expressedby this gene is recognized by anti-sperm agglutinating antibodiesfrom an infertile woman. Furthermore, immunization of female ratswith the recombinant human protein reduced fertility. This proteinlocalizes to the plasma membrane of germ cells in the testis and tothe post-acrosomal plasma membrane of mature spermatozoa.Recombinant polypeptide binds GTP and exhibits GTPase activity.Thus, this protein may regulate GTP signal transduction pathwaysinvolved in spermatogenesis and fertilization. Two transcriptvariants of this gene encode the same protein. [provided byRefSeq].
Background Reference 1:
Lin, W., et al. Mol. Hum. Reprod. 7(9):811-818(2001)
Background Reference 2:
Diekman, A.B., et al. Am. J. Reprod. Immunol. 37(1):111-117(1997)
Background Reference 3:
Zhang, M.L., et al. Chin. Med. J. 105(12):998-1003(1992)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-012

List Size:
400 ul

List Price:

$495.00

Datasheet
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