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Western blot analysis in NCI-H460 cell line lysates (35ug/lane).
Western blot analysis in NCI-H460 cell line lysates (35ug/lane).

SPAG8 Antibody

CATALOG NUMBER: 56-675

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This SPAG8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 383-411 amino acids from the C-terminal region of human SPAG8.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
45 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SPAG8
Additional Names:
Sperm-associated antigen 8, HSD-1, Sperm membrane protein 1, SMP-1, Sperm membrane protein BS-84, SPAG8
Protein Accession Number:
Q99932
Protein Gi Number:
62511128
Ncbi Gene Id Number:
26206
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The correlation of anti-sperm antibodies with cases ofunexplained infertility implicates a role for these antibodies inblocking fertilization. Improved diagnosis and treatment ofimmunologic infertility, as well as identification of proteins fortargeted contraception, are dependent on the identification andcharacterization of relevant sperm antigens. The protein encoded bythis gene is recognized by sperm agglutinating antibodies from aninfertile woman. This protein is localized in germ cells of thetestis at all stages of spermatogenesis and is localized to theacrosomal region of mature spermatozoa. Alternatively splicedvariants that encode different protein isoforms have been describedbut the full-length sequences of only two have been determined.
Background Reference 1:
Wu, H., et al. FEBS Lett. 584(13):2807-2815(2010)
Background Reference 2:
Li, R., et al. Cell Biochem. Funct. 27(5):264-268(2009)
Background Reference 3:
Cheng, G.Y., et al. Asian J. Androl. 9(1):23-29(2007)
Background Reference 4:
Tang, X., et al. J. Mol. Med. 82(6):383-388(2004)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-675

List Size:
400 ul

List Price:

$495.00

Datasheet
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