SPARC Antibody

CATALOG NUMBER: 79-734

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
SPARC antibody was raised against a peptide sequence around aa.52~56 (V-G-E-F-D) derived from Human SPARC.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
Western Blot: 1:500~1:1000
Specificity:
This antibody detects endogenous level of total SPARC protein.
Predicted Molecular Weight:
42 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
SPARC
Additional Names:
ON, ABL, JTK7, ON, BM-40
Protein Accession Number:
NP_003109.1
Protein Gi Number:
4507171
Ncbi Gene Id Number:
6678
Background:
Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca2+ with a low affinity and an EF-hand loop that binds a Ca2+ ion with a high affinity.
Background Reference 1:
Kelm R. J. Jr., Mann K. G. Blood 75:1105-1113 (1990) Wewer U. M., Albrechtsen R., Fisher L. W., Young M. F., Termine J. D. Am. J. Pathol. 132:345-355 (1988) Kuhn C., Mason R. J. Am. J. Pathol. 147:1759-1769 (1995)

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-734

List Size:
0.1 mg

List Price:

$370.00

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