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42-286 (2ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
42-286 (2ug/ml) staining of methanol-fixed HeLa before (left) and after (right) introduction of siRNA.
42-286 (2ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

SPARTIN Antibody

CATALOG NUMBER: 42-286

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-EASGTDVKQLDQGNK
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 70kDa band observed in lysates of cell line HeLa (calculated MW of 72.8kDa according to NP_055902.1). Recommended concentration: 1-3ug/ml.Immunofluorescence: HeLa show dimished signals after knock down of expresssion by introduction of siRNA.
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
SPG20
Additional Names:
SPG20, spastic paraplegia 20 (Troyer syndrome), KIAA0610, SPARTIN, TAHCCP1, OTTHUMP00000018254, spastic paraplegia 20, spastic paraplegia 20, spartin (Troyer syndrome), trans-activated by hepatitis C virus core protein 1
Protein Accession Number:
NP_055902.1
Protein Gi Number:
21703346
Ncbi Gene Id Number:
23111
Background Reference 1:
Lu J, Rashid F, Byrne PC. The hereditary spastic paraplegia protein spartin localises to mitochondria J Neurochem. 2006 Sep;98(6):1908-19.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-286

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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