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Western blot analysis of SPATA6 in A20 cell lysate with SPATA6 antibody at 1 μg/mL.
Immunohistochemistry of SPATA6 in mouse testis tissue with SPATA6 antibody at 2.5 μg/mL.
Immunofluorescence of SPATA6 in mouse testis tissue with SPATA6 antibody at 20 μg/mL.
Western blot analysis of SPATA6 in A20 cell lysate with SPATA6 antibody at 1 μg/mL.
Trial Size

SPATA6 Antibody

CATALOG NUMBER: 6555

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (100%)
Immunogen:
SPATA6 antibody was raised against a 14 amino acid synthetic peptide near the carboxy terminus of human SPATA6.
The immunogen is located within amino acids 400 - 450 of SPATA6.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
SPATA6 antibody can be used for detection of SPATA6 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Specificity:
At least three isoforms of SPATA6 are known to exist; this antibody will detect only the longest two isoforms. SPATA6 antibody is predicted to not cross-react with other SPATA protein family members.
Positive Control 1:
Cat. No. 1288 - A20 Cell Lysate
Purification:
SPATA6 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
SPATA6 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
SPATA6 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SPATA6
Additional Names:
SPATA6 Antibody: HASH, SRF1, SRF-1, Spermatogenesis-associated protein 6
Protein Accession Number:
NP_061946
Protein Gi Number:
9506603
Ncbi Gene Id Number:
54558
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
SPATA6 Antibody: The development of spermatogonial stem cells into spermatozoa, i.e., spermatogenesis, is a highly ordered process that requires multiple proteins, including some that are testis-specific or testis-specific isoforms of genes that are expressed in other tissues. One such protein is SPATA6: while its full-length isoform is expressed in multiple tissues including thymus, brain and testis, the shorter isoform is expressed only in testis. It has some homology to the motor domain of kinesin related proteins and with the C. elegans neural calcium sensor protein NCS-2.
Background Reference 1:
Venables JP. Alternative splicing in the testes. Curr. Opin. Genet. Dev. 2002; 12:615-9.
Background Reference 2:
Oh C, Aho H, Shamsadin R, et al. Characterization, expression pattern and chromosomal localization of the spermatogenesis associated 6 gene (Spata6). Mol. Human Reprod. 2003; 9:321-30.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6555

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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