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Western blot analysis of SPB3 antibody in 293 cell line lysates (35ug/lane)
SPB3 Antibody IHC analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of SPB3 antibody in 293 cell line lysates (35ug/lane)

SPB3 Antibody

CATALOG NUMBER: 62-337

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This SPB3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 40-69 amino acids from the N-terminal region of human SPB3.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
45 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SERPINB3
Additional Names:
Serpin B3, Protein T4-A, Squamous cell carcinoma antigen 1, SCCA-1, SERPINB3, SCCA, SCCA1
Protein Accession Number:
P29508
Protein Gi Number:
20141712
Ncbi Gene Id Number:
6317
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
SPB3 may act as a protease inhibitor to modulate the host immune response against tumor cells.
Background Reference 1:
Turato,C., Dig Liver Dis 41 (3), 212-216 (2009)
Background Reference 2:
Ahmed,S.T., Biochem. Biophys. Res. Commun. 378 (4), 821-825 (2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-337

List Size:
400 ul

List Price:

$495.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

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