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Western blot analysis in K562 cell line lysates (35ug/lane).
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in K562 cell line lysates (35ug/lane).

SPB4 Antibody

CATALOG NUMBER: 55-921

Clonality:
Polyclonal
Tested Applications:
Flow, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This SPB4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 268-296 amino acids from the C-terminal region of human SPB4.
Conjugate:
Unconjugated
Tested Applications:
Flow, WB
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
45 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SERPINB4
Additional Names:
Serpin B4, Leupin, Peptidase inhibitor 11, PI-11, Squamous cell carcinoma antigen 2, SCCA-2, SERPINB4, PI11, SCCA2
Protein Accession Number:
P48594
Protein Gi Number:
1710877
Ncbi Gene Id Number:
6318
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
SPB4 may act as a protease inhibitor to modulate the host immune response against tumor cells.
Background Reference 1:
Murakami, A., et al. Int. J. Oncol. 36(6):1395-1400(2010)
Background Reference 2:
Higgins, W.J., et al. J. Biol. Chem. 285(6):3722-3729(2010)
Background Reference 3:
Villano, G., et al. Int. J. Mol. Med. 25(1):137-143(2010)
Background Reference 4:
Ahmed, S.T., et al. Biochem. Biophys. Res. Commun. 378(4):821-825(2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-921

List Size:
400 ul

List Price:

$495.00

Datasheet
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