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SPEN Antibody

CATALOG NUMBER: 46-426

Clonality:
Polyclonal
Tested Applications:
ELISA
Host Species:
Goat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Homology:
Expected Species Reactivity based on sequence homology: Human
Immunogen:
The immunogen for this antibody is: C-DSEGKMDDKKEDHKE
Conjugate:
Unconjugated
Tested Applications:
ELISA
Application Note:
Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Not yet tested - our routinely used western blotting protocol does not allow detection of proteins as large as the calculated size of approx. 402kDa according to NP_055816.2. Therefore we cannot recommend an optimal concentration and the product is investigative grade. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
SPEN
Additional Names:
SPEN, spen homolog, transcriptional regulator (Drosophila) , KIAA0929, MINT, RP1-134O19.1, SHARP, Msx2 interacting nuclear target (MINT) homolog, OTTHUMP00000044600, SMART/HDAC1 associated repressor protein, nuclear receptor transcription cofactor spen homolog, transcriptional regulator
Protein Accession Number:
NP_055816.2
Protein Gi Number:
14790190
Ncbi Gene Id Number:
23013
Background Reference 1:
Hiriart E, Gruffat H, Buisson M, Mikaelian I, Keppler S, Meresse P, Mercher T, Bernard OA, Sergeant A, Manet E. Interaction of the Epstein-Barr virus mRNA export factor EB2 with human Spen proteins SHARP, OTT1, and a novel member of the family, OTT3, links Spen proteins with splicing regulation and mRNA export. J Biol Chem. 2005 Nov 4;280(44):36935-45. Epub 2005 Aug 29.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-426

List Size:
0.1 mg

List Price:

$391.00

Datasheet
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$65 Standard Overnight flat rate for as many products shipped within the United States.

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