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Western blot analysis of SPG15 in K562 cell lysate with SPG15 antibody at (A) 1 and (B) 2 μg/mL.
Immunofluorescence of SPG15 in Mouse Heart tissue with SPG15 antibody at 20 μg/mL.
Western blot analysis of SPG15 in K562 cell lysate with SPG15 antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

SPG15 Antibody

CATALOG NUMBER: 5023

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (94%)
Immunogen:
SPG15 antibody was raised against a 16 amino acid synthetic peptide near the carboxy terminus of human SPG15.
The immunogen is located within amino acids 1710 - 1760 of SPG15.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, WB
Application Note:
SPG15 antibody can be used for detection of SPG15 by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Specificity:
Multiple isoforms of SPG15 are known to exist.
Positive Control 1:
Cat. No. 1204 - K562 Cell Lysate
Predicted Molecular Weight:
Predicted: 280 kDa
Observed: 260 kDa
Purification:
SPG15 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
SPG15 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
SPG15 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ZFYVE26
Additional Names:
SPG15 Antibody: SPG15, FYVE-CENT, KIAA0321, Zinc finger FYVE domain-containing protein 26, FYVE domain-containing centrosomal protein
Protein Accession Number:
EAW80952
Protein Gi Number:
119601358
Ncbi Gene Id Number:
23503
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
SPG15 Antibody: Hereditary spastic paraplegias (HSPs) are genetically and phenotypically heterogeneous disorders.  Spastic paraplegia with thinning of the corpus callosum (ARHSP-TCC) is a relatively frequent form of complicated hereditary spastic paraplegia in which mental retardation and muscle stiffness at onset are followed by slowly progressive paraparesis and cognitive deterioration. SPG15 is the second gene known to be responsible for ARHSP-TCC in the Italian population. Mutations in this gene are associated with autosomal recessive spastic paraplegia-15. SPG15 encodes a protein containing a FYVE zinc finger binding domain which is thought to target these proteins to membrane lipids through interaction with phospholipids in the membrane. SPG15 mRNA is widely distributed in human tissues, as well as in rat embryos, suggesting a possible role for this protein during embryonic development. SPG15 co-localizes partially with endoplasmic reticulum and endosome markers, suggesting a role in intracellular trafficking.
Background Reference 1:
Hughes CA, Byrne PC, Webb S, et al. SPG15, a new locus for autosomal recessive complicated HSP on chromosome 14q. Neurology 2001; 56:1230-3.
Background Reference 2:
Denora PS, Muglia M, Casali C, et al. Spastic paraplegia with thinning of the corpus callosum and white matter abnormalities: further mutations and relative frequency in ZFYVE26/SPG15 in the Italian population. J. Neurol. Sci. 2009; 277:22-5.
Background Reference 3:
Hanein S, Martin E, Boukhris A, et al. Identification of the SPG15 gene, encoding spastizin, as a frequent cause of complicated autosomal-recessive spastic paraplegia, including Kjellin syndrome. Am. J. Hum. Genet. 2008; 82:992-1002.
Background Reference 4:
Boukhris A, Feki I, Denis E, et al. Spastic paraplegia 15: linkage and clinical description of three Tunisian families. Mov. Disord. 2008; 23:429-33.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5023

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

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