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Western blot analysis of in 293 cell line lysates (35ug/lane)
IHC analysis in formalin fixed and paraffin embedded skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the for immunohistochemistry.
Western blot analysis of SPHK1 using rabbit polyclonal using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SPHK1 gene.
Flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of in 293 cell line lysates (35ug/lane)

SPHK1 Antibody

CATALOG NUMBER: 62-747

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This SPHK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 313-343 amino acids from the C-terminal region of human SPHK1.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
43 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SPHK1
Additional Names:
Sphingosine kinase 1, SK 1, SPK 1, SPHK1, SPHK, SPK
Protein Accession Number:
Q9NYA1
Protein Gi Number:
17369329
Ncbi Gene Id Number:
8877
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
SPHK1 is a novel lipid messenger with both intracellular and extracellular functions. Intracellularly, it regulates proliferation and survival, and extracellularly, it is a ligand for EDG1 (MIM 601974). Various stimuli increase cellular levels of SPP by activation of sphingosine kinase (SPHK), the enzyme that catalyzes the phosphorylation of sphingosine. Competitive inhibitors of SPHK block formation of SPP and selectively inhibit cellular proliferation induced by a variety of factors, including platelet-derived growth factor (e.g., MIM 173430) and serum.
Background Reference 1:
Hengst, J.A., et al. Arch. Biochem. Biophys. 494(1):23-31(2010)
Background Reference 2:
Jarman, K.E., et al. J. Biol. Chem. 285(1):483-492(2010)
Background Reference 3:
Gamble, J.R., et al. Am. J. Pathol. 175(5):2217-2225(2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-747

List Size:
400 ul

List Price:

$495.00

Datasheet
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