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Western blot analysis in HepG2 cell line lysates (35ug/lane).
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.
Western blot analysis in HepG2 cell line lysates (35ug/lane).

SPHK2 Antibody

CATALOG NUMBER: 62-750

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This SPHK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 590-620 amino acids from the C-terminal region of human SPHK2.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
Predicted Molecular Weight:
69 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SPHK2
Additional Names:
Sphingosine kinase 2, SK 2, SPK 2, SPHK2
Protein Accession Number:
Q9NRA0
Protein Gi Number:
22001996
Ncbi Gene Id Number:
56848
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Sphingosine Kinase (SphK) catalyzes the phosphorylation of the lipid sphingosine, creating the bioactive lipid sphingosine-1-phosphate (S1P). S1P subsequently signals through cell surface G protein-coupled receptors, as well as intracellularly, to modulate cell proliferation, survival, motility and differentiation. SphK is an important signaling enzyme which is activated by diverse agents, including growth factors that signal through receptor tyrosine kinases, agents activating G protein-coupled receptors, and immunoglobulin receptors. Two SphK isotypes, SphK-1 and SphK-2, have been cloned, and both isotypes are ubiquitously expressed. SphK-1 has been shown to mediate cell growth, prevention of apoptosis, and cellular transformation, and is upregulated in a variety of human tumors. In contrast, SphK-2 increases apoptosis, and may be responsible for phosphorylating and activating the immunosuppressive drug FTY720.
Background Reference 1:
Shu, X., et al., Mol. Cell. Biol. 22(22):7758-7768 (2002).
Background Reference 2:
Xia, P., et al., J. Biol. Chem. 277(10):7996-8003 (2002).
Background Reference 3:
Liu, H., et al., J. Biol. Chem. 275(26):19513-19520 (2000).
Background Reference 4:
Shutler, G., et al., Genomics 34(3):334-339 (1996).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-750

List Size:
200 ul

List Price:

$495.00

Datasheet
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