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Fluorescent image of NIH/3T3 cell stained with SPI1 Antibody .NIH/3T3 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with SPI1 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml). SPI1 immunoreactivity is localized to Nucleus significantly.
Western blot analysis in WiDr cell line lysates (35ug/lane).
SPI1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Fluorescent image of NIH/3T3 cell stained with SPI1 Antibody .NIH/3T3 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with SPI1 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml). SPI1 immunoreactivity is localized to Nucleus significantly.

SPI1 Antibody

CATALOG NUMBER: 58-037

Clonality:
Polyclonal
Tested Applications:
IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Pig, Rat
Immunogen:
This SPI1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 217-246 amino acids from the C-terminal region of human SPI1.
Conjugate:
Unconjugated
Tested Applications:
IF, IHC-P, WB
Application Note:
For IF starting dilution is: 1:10~50
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
31 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SPI1
Additional Names:
Transcription factor PU1, 31 kDa-transforming protein, SPI1
Protein Accession Number:
P17947
Protein Gi Number:
60415923
Ncbi Gene Id Number:
6688
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes an ETS-domain transcription factor thatactivates gene expression during myeloid and B-lymphoid celldevelopment. The nuclear protein binds to a purine-rich sequenceknown as the PU-box found near the promoters of target genes, andregulates their expression in coordination with other transcriptionfactors and cofactors. The protein can also regulate alternativesplicing of target genes. Multiple transcript variants encodingdifferent isoforms have been found for this gene. [provided byRefSeq].
Background Reference 1:
Rimmele, P., et al. Cancer Res. 70(17):6757-6766(2010)
Background Reference 2:
Bonadies, N., et al. Oncogene 29(7):1062-1072(2010)
Background Reference 3:
Bonadies, N., et al. Blood 115(2):331-334(2010)
Background Reference 4:
Desai, S., et al. J. Immunol. 183(9):5778-5787(2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

58-037

List Size:
400 ul

List Price:

$495.00

Datasheet
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