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46-429 staining (0.5ug/ml) of Human Brain (Cerebral Cortex) lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.
46-429 (5ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
46-429 (5ug/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
46-429 staining (0.5ug/ml) of Human Brain (Cerebral Cortex) lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.

Spinesin Antibody

CATALOG NUMBER: 46-429

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Dog
Immunogen:
The immunogen for this antibody is: C-LDWIHDTAQDSLL
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Experiments gave bands at approx 50kDa and 29kDa in Human Brain (Cerebral Cortex). These bands correspond to earlier findings in literature with different antibodies (Yamaguchi et al, J Biol Chem. 2002 Mar 1;277(9):6806-12; PMID: 11741986). This protein has a calculated MW of 49.6kDa according to NP_110397.1. Recommended concentration: 0.5-1.5ug/ml.Immunohistochemistry:Paraffin embedded Human Liver and Small Intestine. Recommended concentration: 5ug/ml.
Positive Control 1:
Cat. No. XBL-10106 - Human Cerebral Cortex Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
TMPRSS5
Additional Names:
TMPRSS5, SPINESIN, transmembrane protease, serine 5 (spinesin), MGC141886, MGC148044, SPINESIN
Protein Accession Number:
NP_110397.2
Protein Gi Number:
13540535
Ncbi Gene Id Number:
80975
Background Reference 1:
Yamaguchi N, Okui A, Yamada T, Nakazato H, Mitsui S. Spinesin/TMPRSS5, a novel transmembrane serine protease, cloned from human spinal cord. J Biol Chem. 2002 Mar 1;277(9):6806-12.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-429

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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