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Western blot analysis of SPINK2 in human testis tissue lysate with SPINK2 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Immunohistochemistry of SPINK2 in mouse testis tissue with SPINK2 antibody at 2.5 μg/mL.
Immunofluorescence of SPINK2 in mouse testis tissue with SPINK2 antibody at 20 μg/mL.
Western blot analysis of SPINK2 in human testis tissue lysate with SPINK2 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Trial Size

SPINK2 Antibody

CATALOG NUMBER: 6667

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Rat: (100%)
Immunogen:
SPINK2 antibody was raised against a 16 amino acid synthetic peptide near the carboxy terminus of mouse SPINK2.
The immunogen is located within the last 50 amino acids of SPINK2.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
SPINK2 antibody can be used for detection of SPINK2 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Specificity:
SPINK2 antibody is predicted to not cross-react with other SPINK family members.
Positive Control 1:
Cat. No. 1313 - Human Testis Tissue Lysate
Predicted Molecular Weight:
Predicted: 15 kDa
Observed: 18 kDa
Purification:
SPINK2 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
SPINK2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
SPINK2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SPINK2
Additional Names:
SPINK2 Antibody: HUSI-II, Serine protease inhibitor Kazal-type 2, Acrosin-trypsin inhibitor
Protein Accession Number:
NP_001276693.1
Protein Gi Number:
576796017
Ncbi Gene Id Number:
69982
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
SPINK2 Antibody: Human serine proteinase inhibitor Kazal-type 2 (SPINK2) is required for maintaining normal spermatogenesis and potentially regulates serine protease-mediated apoptosis in male germ cells. It contains a typical kazal domain composed by six cysteine residues forming three disulfide bridges. SPINK2 functions as a trypsin/acrosin inhibitor and is synthesized mainly in the testis and seminal vesicle where its activity is engaged in fertility. SPINK2 plays a role in the pathogenesis of hereditary and chronic pancreatitis.
Background Reference 1:
Lee B, Park I, Jin S, et al. Impaired spermatogenesis and fertility in mice carrying a mutation in the Spink2 gene expressed predominantly in testes. J. Biol. Chem. 2011; 286:29108-17
Background Reference 2:
Chen T, Lee TR, Liang WG, et al. Identification of trypsin-inhibitory site and structure determination of human SPINK2 serine proteinase inhibitor. Proteins 2009; 77:209-19.
Background Reference 3:
Liddle RA. Pathophysiology of SPINK mutations in pancreatic development and disease. Endocrinol. Metab. Clin. North Am. 2006; 35:345-56.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6667

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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