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Western blot analysis of SPINK5 Antibody in A2058 cell line lysates (35ug/lane)
SPINK5 Antibody (RB18954) IHC analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of A2058 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of SPINK5 Antibody in A2058 cell line lysates (35ug/lane)

SPINK5 Antibody

CATALOG NUMBER: 62-512

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This SPINK5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 188-217 amino acids from the N-terminal region of human SPINK5.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
121 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SPINK5
Additional Names:
Serine protease inhibitor Kazal-type 5, Lympho-epithelial Kazal-type-related inhibitor, LEKTI, Hemofiltrate peptide HF6478, Hemofiltrate peptide HF7665, SPINK5
Protein Accession Number:
Q9NQ38
Protein Gi Number:
212276440
Ncbi Gene Id Number:
11005
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
SPINK5 is a multidomain serine protease inhibitor that contains 15 potential inhibitory domains. The inhibitor may play a role in skin and hair morphogenesis and anti-inflammatory and/or antimicrobial protection of mucous epithelia. Mutations may result in Netherton syndrome, a disorder characterized by ichthyosis, defective cornification, and atopy.
Background Reference 1:
Nin,M., et.al., J. Dermatol. Sci. 54 (1), 17-24 (2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-512

List Size:
400 ul

List Price:

$495.00

Datasheet
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