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Western blot analysis of SPINSTER in HepG2 cell lysate with SPINSTER antibody at (A) 1 and (B) 2 μg/ml.
Immunohistochemistry of SPINSTER in human lung tissue with SPINSTER antibody at 2.5 μg/ml.
Immunofluorescence of SPINSTER in human lung tissue with SPINSTER antibody at 20 μg/ml.
Western blot analysis of SPINSTER in HepG2 cell lysate with SPINSTER antibody at (A) 1 and (B) 2 μg/ml.
Trial Size

SPINSTER Antibody

CATALOG NUMBER: 7651

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (83%), Rat: (81%), Mouse: (81%)
Immunogen:
SPINSTER antibody was raised against an 18 amino acid peptide near the amino terminus of human SPINSTER.
The immunogen is located within the first 50 amino acids of SPINSTER.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
SPINSTER antibody can be used for detection of SPINSTER by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
SPINSTER antibody is human specific. At least four isoforms of SPINSTER are known to exist. This antibody is predicted to not cross-react with other members of the spinster family of proteins.
Positive Control 1:
Cat. No. 1211 - HepG2 Cell Lysate
Positive Control 2:
Cat. No. 1309 - Human Placenta Tissue Lysate
Positive Control 3:
Cat. No. 10-101 - Human Lung Tissue Slide
Predicted Molecular Weight:
Predicted: 58 kDa
Observed: 56 kDa
Purification:
SPINSTER antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
SPINSTER antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
SPINSTER antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
SPNS1
Additional Names:
SPINSTER Antibody: LAT, nrs, SPIN1, SPINL, HSpin1, PP2030, PP20300, Protein spinster homolog 1
Protein Accession Number:
NP_001135920
Protein Gi Number:
215490098
Ncbi Gene Id Number:
83985
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
SPINSTER, also known as SPNS1 or SPIN1, is a 528 amino acid multi-pass membrane protein that localizes to the inner mitochondrial membrane and belongs to the spinster subfamily of the major facilitator superfamily (1). SPINSTER interacts with Bcl-x and Bcl-2 and, via this interaction, is thought to be involved in necrotic or autophagic cell death (2). The related protein SPNS2 is critical for the normal lymphocyte localization and mammalian immune system function (1,3).
Background Reference 1:
Saier MH Jr, Beatty JT, Goffeau A, et al. The major facilitator superfamily. J. Mol. Microbiol. Biotechnol. 1999; 1:257-79.
Background Reference 2:
Yanagisawa H, Miyashita T, Nakano Y, et al. HSpin1, a transmembrane protein interacting with Bcl-2/Bcl-xL, induces a caspase-independent autophagic cell death. Cell Death Differ. 2003; 10:798-807.
Background Reference 3:
Nakano Y, Fujitani K, Kurihara J, et al. Mutations in the novel membrane protein spinster interfere with programmed cell death and cause neural degeneration in Drosophila melanogaster. Mol. Cell. Biol. 2001; 21:3775-88.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7651

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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$65 Standard Overnight flat rate for as many products shipped within the United States.

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