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Western blot analysis of extracts of various cell lines, using SPINT1 antibody (22-201) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human prostate using SPINT1 antibody (22-201) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse esophageal cancer using SPINT1 antibody (22-201) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using SPINT1 antibody (22-201). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using SPINT1 antibody (22-201) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 90s.

SPINT1 Antibody

CATALOG NUMBER: 22-201

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 234-513 of human SPINT1 (NP_003701.1).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:100
IF: 1:50 - 1:100
Positive Control 1:
Mouse liver
Positive Control 2:
Rat brain
Predicted Molecular Weight:
Observed: 70kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
SPINT1
Additional Names:
Kunitz-type protease inhibitor 1, Hepatocyte growth factor activator inhibitor type 1, HAI-1, SPINT1, HAI1
Ncbi Gene Id Number:
6692
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a member of the Kunitz family of serine protease inhibitors. The protein is a potent inhibitor specific for HGF activator and is thought to be involved in the regulation of the proteolytic activation of HGF in injured tissues. Alternative splicing results in multiple variants encoding different isoforms.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

22-201

List Size:
100 uL

List Price:

$436.00

Datasheet
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