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Western blot analysis in WiDr cell line lysates (35ug/lane).
Western blot analysis in WiDr cell line lysates (35ug/lane).

SPOP Antibody

CATALOG NUMBER: 60-100

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Xenopus, Bovine, Zebrafish, Mouse
Immunogen:
This SPOP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 86-115 amino acids from the N-terminal region of human SPOP.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
42 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SPOP
Additional Names:
Speckle-type POZ protein, HIB homolog 1, Roadkill homolog 1, SPOP
Protein Accession Number:
O43791
Ncbi Gene Id Number:
8405
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a protein that may modulate thetranscriptional repression activities of death-associated protein 6(DAXX), which interacts with histone deacetylase, core histones,and other histone-associated proteins. In mouse, the encodedprotein binds to the putative leucine zipper domain of macroH2A1.2,a variant H2A histone that is enriched on inactivated Xchromosomes. The BTB/POZ domain of this protein has been shown inother proteins to mediate transcriptional repression and tointeract with components of histone deacetylase co-repressorcomplexes. Alternative splicing of this gene results in multipletranscript variants encoding the same protein. [provided byRefSeq].
Background Reference 1:
Rose, J. Phd, et al. Mol. Med. (2010) In press :
Background Reference 2:
Zhuang, M., et al. Mol. Cell 36(1):39-50(2009)
Background Reference 3:
Liu, J., et al. Science 323(5918):1218-1222(2009)
Background Reference 4:
Bunce, M.W., et al. J. Biol. Chem. 283(13):8678-8686(2008)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

60-100

List Size:
400 ul

List Price:

$469.00

Out of stock

Datasheet
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