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Western blot analysis in uterus tumor cell line lysates (35ug/lane).
SPOPL Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in uterus tumor cell line lysates (35ug/lane).

SPOPL Antibody

CATALOG NUMBER: 56-633

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This SPOPL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 221-249 amino acids from the Central region of human SPOPL.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
45 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SPOPL
Additional Names:
Speckle-type POZ protein-like, HIB homolog 2, Roadkill homolog 2, SPOPL
Protein Accession Number:
Q6IQ16
Protein Gi Number:
74736582
Ncbi Gene Id Number:
339745
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
In complex with a cullin, may act in ubiquitination and proteasomal degradation processes (By similarity).
Background Reference 1:
Lamesch, P., et al. Genomics 89(3):307-315(2007)
Background Reference 2:
Zhang, Q., et al. Dev. Cell 10(6):719-729(2006)
Background Reference 3:
Kent, D., et al. Development 133(10):2001-2010(2006)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-633

List Size:
400 ul

List Price:

$495.00

Datasheet
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