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Western blot analysis of SPP1 in human bladder tissue lysate with SPP1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide
Immunohistochemistry of SPP1 in human bladder tissue with SPP1 antibody at 5 μg/mL.
Immunofluorescence of SPP1 in human bladder tissue with SPP1 antibody at 20 μg/mL.
Western blot analysis of SPP1 in human bladder tissue lysate with SPP1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide
Trial Size

SPP1 Antibody

CATALOG NUMBER: 7443

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Pig: (94%), Bovine: (83%)
Immunogen:
SPP1 antibody was raised against an 18 amino acid peptide near the amino terminus of human SPP1.
The immunogen is located within amino acids 50 - 100 of SPP1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
SPP1 antibody can be used for detection of SPP1 by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
SPP1 antibody is human, mouse and rat reactive. Multiple isoforms of SPP1 are known to exist.
Positive Control 1:
Cat. No. 1310 - Human Bladder Tissue Lysate
Predicted Molecular Weight:
Predicted: 36 kDa
Observed: 37 kDa
Purification:
SPP1 Antibody is Protein A purified.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
SPP1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
SPP1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
SPP1
Additional Names:
SPP1 Antibody: OPN, BNSP, BSPI, ETA-1, OPN, PSEC0156, Osteopontin, Bone sialoprotein 1, SPP-1
Protein Accession Number:
NP_001238759
Protein Gi Number:
352962176
Ncbi Gene Id Number:
6696
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
SPP1 Antibody: The secreted protein 1 (SPP1), also known as osteopontin, is a major noncollagenous protein of bone, but is also found in the extracellular matrix of other mineralized tissues and in bodily fluids. In bone, SPP1 is produced by osteoblasts, osteocytes, macrophages, and osteoclasts (1,2). SPP1 binds to cells through integrin and non-integrin receptors, as well as the adhesion receptor CD44 in an RGD-independent manner, enabling SPP1 to induce a number of functional effects including macrophage chemotaxis, cytoprotection, and regulation of T helper type 1 cells (2). SPP1 can regulate biomineralization by inhibiting the formation of hydroxyapatite (3) and the growth of calcium oxalate crystals (4).
Background Reference 1:
Heinegard D, Andersson G, and Reinholt FP. Roles of osteopontin in bone remodeling. Ann. N.Y. Acad. Sci.1995; 760:213-22.
Background Reference 2:
Wang KX and Denhardt DT. Osteopontin: rule in immune regulation and stress responses. Cyto. Growth Factor Rev. 2008; 19:333-45.
Background Reference 3:
Boskey AL, Maresca M, Ullrich W, et al. Osteopontin-hydroxyapatite interactions in vitro: inhibition of hydroxyapatite formation and growth in a gelatin-gel. Bone Miner. 1993; 22:147-159.
Background Reference 4:
Shiraga H, Min W, VanDusen WJ, et al. Inhibition of calcium oxalate crystal growth in vitro by uropontin: another member of the aspartic acid-rich protein superfamily. Proc. Natl. Acad. Sci. USA 1992; 89:426-30.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7443

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

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