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Western blot analysis of Spred2 in human small intestine tissue lysate with Spred2 antibody at (A) 1 and (B) 2 μg/mL.
Western blot analysis of Spred2 in human small intestine tissue lysate with Spred2 antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

Spred2 Antibody

CATALOG NUMBER: 4849

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse: (85%), Rat: (85%)
Immunogen:
Spred2 antibody was raised against a 13 amino acid synthetic peptide near the center of the human Spred2.
The immunogen is located within amino acids 140 - 190 of Spred2.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Spred2 antibody can be used for detection of Spred2 by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
Specificity:
This Spred2 antibody is predicted to have no cross-reactivity to Spred1 or Spred3.
Positive Control 1:
Cat. No. 1308 - Human Small Intestine Tissue Lysate
Purification:
Spred2 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Spred2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
Spred2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SPRED2
Additional Names:
Spred2 Antibody: Spred-2, Sprouty-related, EVH1 domain-containing protein 2, Spred-2
Protein Accession Number:
NP_861449
Protein Gi Number:
189571669
Ncbi Gene Id Number:
200734
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Spred2 Antibody: Spred2 is a member of the Sprouty family, a group of proteins that act as negative regulators during development. Like Spred1, Spred2 acts by suppressing the phosphorylation and activation of Raf. The Spred proteins have also been implicated in the negative feedback regulation of FGF signaling in embryogenesis and angiogenesis. Further studies have shown that expression levels of Spred1 and Spred2 proteins are inversely correlated with the incidence of tumor invasion and metastasis in human hepatocellular carcinoma (HHC), suggesting that these proteins could be useful as prognostic factors and therapeutic targets in HCC. At least two isoforms of Spred2 are known to exist.
Background Reference 1:
Wakioka T, Sasaki A, Kato R, et al. Spred is a Sprouty-related suppressor of Ras signalling. Nature2001; 412:647-51.
Background Reference 2:
Casci T, Vinos J, and Freeman M. Sprouty, an intracellular inhibitor of Ras signaling. Cell1999; 96:655-65.
Background Reference 3:
Minowada G, Jarvis LA, Chi CL, et al. Vertebrate Sprouty genes are induced by FGF signaling and can cause chondrodysplasia when overexpressed. Development1999; 126:4465-75.
Background Reference 4:
Lee SH, Schloss DJ, Jarvis L, et al. Inhibition of angiogenesis by a mouse sprouty protein. J. Biol. Chem.2001; 276:4128-33.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

4849

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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