Start Your Project

🛍️Explore our Promotions page to discover current deals, limited-time offers, and more!🌟

Western blot analysis of Spred3 in mouse brain tissue lysate with Spred3 antibody at (A) 2 and (B) 4 μg/mL.
Immunohistochemistry of Spred3 in human brain tissue with Spred3 antibody at 2.5 μg/mL.
Immunofluorescence of spred3 in human brain tissue with spred3 antibody at 20 μg/mL.
Western blot analysis of Spred3 in mouse brain tissue lysate with Spred3 antibody at (A) 2 and (B) 4 μg/mL.
Trial Size

Spred3 Antibody

CATALOG NUMBER: 4851

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Spred3 antibody was raised against a 16 amino acid synthetic peptide near the center of the human Spred3.
The immunogen is located within amino acids 140 - 190 of Spred3.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
Spred3 antibody can be used for detection of Spred3 by Western blot at 2 - 4 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
This Spred3 antibody is predicted to have no cross-reactivity to Spred1 or Spred2.
Positive Control 1:
Cat. No. 1403 - Mouse Brain Tissue Lysate
Positive Control 2:
Cat. No. 10-301 - Human Brain Tissue Slide
Predicted Molecular Weight:
Predicted: 45 kDa
Observed: 45 kDa
Purification:
Spred3 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Spred3 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
Spred3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SPRED3
Additional Names:
Spred3 Antibody: Eve-3, spred-3, EVE-3, Sprouty-related, EVH1 domain-containing protein 3, Spred-3
Protein Accession Number:
NP_001035987
Protein Gi Number:
110225353
Ncbi Gene Id Number:
399473
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Spred3 Antibody: Spred3 is a member of the Sprouty family, a group of proteins that act as negative regulators during development. Like Spred1 and Spred2, Spred3 contains and EVH1 domain in its amino terminus and a Sprouty-related cysteine-rich (SPR) domain in its C-terminus, but does not possess a functional c-kit binding domain (KBD). The Spred proteins have also been implicated in the negative feedback regulation of FGF signaling in embryogenesis and angiogenesis. Although Spred3 was initially reported to be expressed in brain, at least one report also indicates that Spred3 can be found in liver. At least two isoforms of Spred3 are known to exist.
Background Reference 1:
Kato R, Nonami A, Taketomi T, et al. Molecular cloning of mammalian Spred-3 which suppresses tyrosine kinase-mediated Erk activation. Biochem. Biophys. Res. Commun.2003; 302:767-72.
Background Reference 2:
Casci T, Vinos J, and Freeman M. Sprouty, an intracellular inhibitor of Ras signaling. Cell1999; 96:655-65.
Background Reference 3:
Minowada G, Jarvis LA, Chi CL, et al. Vertebrate Sprouty genes are induced by FGF signaling and can cause chondrodysplasia when overexpressed. Development1999; 126:4465-75.
Background Reference 4:
Lee SH, Schloss DJ, Jarvis L, et al. Inhibition of angiogenesis by a mouse sprouty protein. J. Biol. Chem.2001; 276:4128-33.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

4851

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

Customize your order

Bulk Pricing Available

Custom Formulations: azide free, endotoxin testing & endotoxin removal.

Pair your antibody with a Lysate, Peptide, Control, & /OR Secondary Antibody.

Contact our Customer Care Team for more information

Custom Antibody Services

Speak to one of our custom antibody specialists to discuss your next custom antibody project.

New & Featured Products

HIV-1 p24 Antibody [9D2F6] (HRP)
HIV-1 p24 Antibody [9D2F6] (HRP)

CATALOG NUMBER: PM-7369-HRP

HIV-1 p24 Antibody [9D2F6] (biotin)
HIV-1 p24 Antibody [9D2F6] (biotin)

CATALOG NUMBER: PM-7369-biotin

HIV-1 p24 Antibody [9D2F6]
HIV-1 p24 Antibody [9D2F6]

CATALOG NUMBER: PM-7369