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Western blot analysis of anti-SPRY4 Antibody in Sk-Br-3 cell line lysates (35ug/lane)
Western blot analysis of anti-SPRY4 Antibody in Sk-Br-3 cell line lysates (35ug/lane)

SPRY4 Antibody

CATALOG NUMBER: 62-914

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This SPRY4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 270-301 amino acids from the C-terminal region of human SPRY4.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
33 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SPRY4
Additional Names:
Protein sprouty homolog 4, Spry-4, SPRY4
Protein Accession Number:
Q9C004
Protein Gi Number:
14916719
Ncbi Gene Id Number:
81848
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
SPRY4 is an inhibitor of the receptor-transduced mitogen-activated protein kinase (MAPK) signaling pathway. The protein is positioned upstream of RAS activation and impairs the formation of active GTP-RAS.
Background Reference 1:
Tsai CF, et al. (2008)Proteome Res
Background Reference 2:
Guo A, et al. (2008) Proc Natl Acad SciS105, 692-7
Background Reference 3:
Rikova K, et al. (2007) Cell 131, 1190-203
Background Reference 4:
Wolf-Yadlin A, Hautaniemi S, Lauffenburger DA, White FM (2007) Proc Natl Acad SciS104, 5860-5

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-914

List Size:
400 ul

List Price:

$495.00

Datasheet
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