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Western blot analysis of SPRYD2 in mouse heart tissue lysate with SPRYD2 antibody at 1 μg/mL.
Immunohistochemistry of SPRYD2 in mouse brain tissue with SPRYD2 antibody at 2.5 μg/mL.
Immunofluorescence of SPRYD2 in mouse brain tissue with SPRYD2 antibody at 20 μg/mL.
Western blot analysis of SPRYD2 in mouse heart tissue lysate with SPRYD2 antibody at 1 μg/mL.
Trial Size

SPRYD2 Antibody

CATALOG NUMBER: 6403

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
SPRYD2 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human SPRYD2.
The immunogen is located within amino acids 3810 - 3860 of SPRYD2.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
SPRYD2 antibody can be used for detection of SPRYD2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Specificity:
SPRYD2 antibody is predicted to not cross-react with other SPRYD protein family members. At least four isoforms of SPRYD2 are known to exist.
Positive Control 1:
Cat. No. 1463 - Rat Brain Tissue Lysate
Purification:
SPRYD2 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
SPRYD2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
SPRYD2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CMYA5
Additional Names:
SPRYD2 Antibody: SPRYD2, TRIM76, C5orf10, DTNBP2, SPRYD2, Cardiomyopathy-associated protein 5, Dystrobrevin-binding protein 2
Protein Accession Number:
NP_705838
Protein Gi Number:
62241003
Ncbi Gene Id Number:
202333
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
SPRYD2 Antibody: SPRYD2, also known as Myospryn, was originally identified as the muscle-specific partner of dysbindin and as a Mef-2 target gene. It is a large scaffolding protein localized to the Z-disc/costamere region of striated muscle. SPRYD2 includes a noncanonical tripartite motif (TRIM-like) that lacks the RING domain but consists of a B-box coiled coil (BBC), fibronectin 3 (FN3) repeats, and SPRY domains. SPRYD2 interacts with desmin and calcineurin, and it has been suggested to play a role in the biogenesis of lysosome and negatively regulates slow-fiber-type transformation and skeletal muscle regeneration. SPRYD2 is dysregulated in Duchenne muscular dystrophy.
Background Reference 1:
Benson MA, Tinsley CL, Blake DJ. Myospryn is a novel binding partner for dysbindin in muscle. J. Biol. Chem. 2004; 279:10450-8.
Background Reference 2:
Sarparanta J. Biology of myospryn: what's known? J. Muscle Res. Cell Motil. 2008; 29:177-80
Background Reference 3:
Durham JT, Brand OM, Arnold M, et al. Myospryn is a direct transcriptional target for MEF2A that encodes a striated muscle, alpha-actinin-interacting, costamere-localized protein. J. Biol. Chem. 2006; 281:6841-9
Background Reference 4:
Kielbasa OM, Reynolds JG, Wu CL, et al. Myospryn is a calcineurin-interacting protein that negatively modulates slow-fiber-type transformation and skeletal muscle regeneration. FASEB J. 2011 epub.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6403

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

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