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Western blot analysis of SPRYD3 in human brain tissue lysate with SPRYD3 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide
Immunohistochemistry of SPRYD3 in mouse brain tissue with SPRYD3 antibody at 5 μg/mL.
Immunofluorescence of SPRYD3 in mouse brain tissue with SPRYD3 antibody at 20 μg/mL.
Western blot analysis of SPRYD3 in human brain tissue lysate with SPRYD3 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide
Trial Size

SPRYD3 Antibody

CATALOG NUMBER: 6405

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
SPRYD3 antibody was raised against a 16 amino acid synthetic peptide near the center of human SPRYD3.
The immunogen is located within amino acids 260 - 310 of SPRYD3.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
SPRYD3 antibody can be used for detection of SPRYD3 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Specificity:
SPRYD3 antibody is predicted to not cross-react with other SPRYD protein family members. At least two isoforms of SPRYD3 are known to exist.
Positive Control 1:
Cat. No. 1303 - Human Brain Tissue Lysate
Purification:
SPRYD3 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
SPRYD3 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
SPRYD3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SPRYD3
Additional Names:
SPRYD3 Antibody: SPRY domain-containing protein 3
Protein Accession Number:
NP_116229
Protein Gi Number:
14249554
Ncbi Gene Id Number:
84926
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
SPRYD3 Antibody: The SPRY domain-containing protein 3 (SPRYD3) is a member of a family of proteins whose sole common characteristic is the presence of a SPRY domain. SPRY domains are structural domains that were first described in the fungal Dictyostelium discoideum tyrosine kinase spore lysis A. In most systems SPRY domains provide binding sites for regulatory proteins or intramolecular binding sites that maintain the structural integrity of a protein. Little is known of the function of the SPRYD3 protein.
Background Reference 1:
Tae H, Casarotto MG, and Dulhunty AF. Ubiquitous SPRY domains and their role in the skeletal type ryanodine receptor. Eur. Biophys. J. 2009; 39:51-9.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6405

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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