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Western Blot at 1:2000 dilution Lane 1: human heart lysate Lane 2: human kidney lysate Lane 3: human uterus lysate Lysates/proteins at 20 ug per lane.
Western Blot at 1:2000 dilution Lane 1: human heart lysate Lane 2: human kidney lysate Lane 3: human uterus lysate Lysates/proteins at 20 ug per lane.

SPTLC3 Antibody

CATALOG NUMBER: 61-312

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This SPTLC3 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 503-545 amino acids from human SPTLC3.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:2000
Predicted Molecular Weight:
62 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SPTLC3
Additional Names:
Serine palmitoyltransferase 3, 2.3.1.50, Long chain base biosynthesis protein 2b, LCB2b, Long chain base biosynthesis protein 3, LCB 3, Serine-palmitoyl-CoA transferase 3, SPT 3, SPTLC3, C20orf38, SPTLC2L
Protein Accession Number:
Q9NUV7
Protein Gi Number:
158931158
Ncbi Gene Id Number:
55304
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Serine palmitoyltransferase (SPT). The heterodimer formed with LCB1/SPTLC1 constitutes the catalytic core. The composition of the serine palmitoyltransferase (SPT) complex determines the substrate preference. The SPTLC1-SPTLC3-SPTSSA isozyme uses both C14-CoA and C16-CoA as substrates, while the SPTLC1-SPTLC3-SPTSSB has the ability to use a broader range of acyl-CoAs without apparent preference.
Background Reference 1:
Hornemann T.,et al.J. Biol. Chem. 281:37275-37281(2006).
Background Reference 2:
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Background Reference 3:
Deloukas P.,et al.Nature 414:865-871(2001).
Background Reference 4:
Han G.,et al.Proc. Natl. Acad. Sci. U.S.A. 106:8186-8191(2009).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-312

List Size:
200 ul

List Price:

$495.00

Datasheet
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