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Western blot analysis using SRA monoclonal antibody against truncated SRA recombinant protein and human ovary cancer tissue lysate .
Immunohistochemical analysis of paraffin - embedded human bladder carcinoma (top) and breast carcinoma (bottom) showing nuclear and cytoplasmic location using SRA antibody with DAB staining.
Western blot analysis using SRA monoclonal antibody against truncated SRA recombinant protein and human ovary cancer tissue lysate .

SRA Antibody [7H1G1]

CATALOG NUMBER: 32-220

Clonality:
Monoclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Immunogen:
Ni-NTA purified truncated recombinant SRA expressed in E. Coli strain BL21 (DE3).
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Western Blot:1:500 - 1:2,000
IHF(P):1:500 - 1:2,000
IHC(F):1:500 - 1:2,000
ELISA:Propose dilution 1:10,000
Determining optimal working dilutions by titration test.
Clonality:
Monoclonal
Isotype:
IgG1
Buffer:
Ascitic fluid containing 0.03% sodium azide.
Concentration:
batch dependent
Storage Conditions:
SRA monoclonal antibody can be stored at -20°C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SRA1
Additional Names:
Steroid receptor RNA activator protein, SRAP, SRA, STRAA1, pp7684
Protein Accession Number:
Q9HD15
Protein Gi Number:
74718904
Ncbi Gene Id Number:
10011
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Steroid receptor RNA activator 1 (SRA), with 237-amino acid protein (about 27 kDa), belongs to the growing family of functional non-coding RNAs. SRA was originally described as the first functional noncoding RNA able to specifically coactivate the activity of steroid receptors. Specifically, SRA exists as both an RNA transcript that forms a complex with steroid receptor coactivator-1 and as a stably expressed protein. Its expression is strongly up-regulated in many human tumors of the breast, uterus, and ovary, suggesting a potential role in pathogenesis. Although coactivation of steroid-dependent transcription by SRA is accompanied by a proliferative response, overexpression is not in itself sufficient to induce turmorigenesis.
Background Reference 1:
Rainer B. Lanz, Steven S. Chua, Niall Barron. Mol. Cell. Biol, Oct 2003; 23: 7163 - 7176.
Background Reference 2:
Shilpa Chooniedass-Kothari, Mohammad Kariminia Hamedani, Sandy Troup. Int J Cancer. 2006 Feb 15;118(4):1054-9
Background Reference 3:
S. Chooniedass-Kothari, E. Emberley, M. K. Hamedani. FEBS Lett. 2004 May 21;566(1-3):43-7.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

32-220

List Size:
0.1 mL

List Price:

$432.00

Datasheet
Shipping Info

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$35 Dry Ice Shipment fee may be required on some items.

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