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Western blot analysis in CEM cell line lysates (35ug/lane).
Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with SRC1 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot analysis in CEM cell line lysates (35ug/lane).

SRC1 Antibody

CATALOG NUMBER: 63-002

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Pig
Immunogen:
This SRC1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1338-1367 amino acids from the C-terminal region of human SRC1.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
157 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
NCOA1
Additional Names:
Nuclear receptor coactivator 1, NCoA-1, Class E basic helix-loop-helix protein 74, bHLHe74, Protein Hin-2, RIP160, Renal carcinoma antigen NY-REN-52, Steroid receptor coactivator 1, SRC-1, NCOA1, BHLHE74, SRC1
Protein Accession Number:
Q15788
Protein Gi Number:
158518533
Ncbi Gene Id Number:
8648
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
SRC1 acts as a transcriptional coactivator for steroid and nuclear hormone receptors. It is a member of the p160/steroid receptor coactivator (SRC) family and like other family members has histone acetyltransferase activity and contains a nuclear localization signal, as well as bHLH and PAS domains. This protein binds nuclear receptors directly and stimulates the transcriptional activities in a hormone-dependent fashion.
Background Reference 1:
Lavery,D.N.,Biochemistry 47 (11), 3352-3359 (2008)
Background Reference 2:
Wang,S., J. Biol. Chem. 282 (5), 2765-2775 (2007)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-002

List Size:
400 ul

List Price:

$495.00

Datasheet
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