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42-210 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. The nuclear stain is DAPI (blue)
42-210 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. The nuclear stain is DAPI (blue)

SRCAP Antibody

CATALOG NUMBER: 42-210

Clonality:
Polyclonal
Tested Applications:
ELISA, IF
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog
Immunogen:
The immunogen for this antibody is: C-DEAQNIKNFKSQR
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Not yet tested - our routine western blotting protocol does not allow for the detection of proteins >250kDa (calculated size of approx. 344kDa according to NP_006653.2).Immunofluorescence: Strong expression of the protein seen in the Nucleus of A31 cells. Recommended concentration: 10ug/ml.
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
SRCAP
Additional Names:
SRCAP, Snf2-related CREBBP activator protein, DOMO1, EAF1, FLJ44499, KIAA0309, SWR1, OTTHUMP00000163226, Snf2-related CBP activator protein, Swi2/Snf2-related ATPase homolog, domino homolog 1
Protein Accession Number:
NP_006653.2
Protein Gi Number:
146219843
Ncbi Gene Id Number:
10847
Background Reference 1:
Wong MM, Cox LK, Chrivia JC. The chromatin remodeling protein, SRCAP, is critical for deposition of the histone variant H2A.Z at promoters. The Journal of biological chemistry 2007 Sep 282 (36): 26132-9.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-210

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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