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Western blot analysis of various lysates using SRD5A2 Rabbit mAb at 1:7000 dilution incubated overnight at 4℃. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 45 s.
Western blot analysis of various lysates using SRD5A2 Rabbit mAb at 1:7000 dilution incubated overnight at 4℃. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 45 s.

SRD5A2 Rabbit Recombinant Antibody (81-624)

CATALOG NUMBER: 81-624

Clonality:
Monoclonal
Tested Applications:
ELISA, IF-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF-P, WB
Application Note:
WB,1:1000 - 1:2000, IF-P,1:100 - 1:400, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Positive Control 1:
PC-3,Mouse liver,Mouse testis,Mouse kidney,Rat testis,Rat kidney
Predicted Molecular Weight:
28kDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
SRD5A2
Protein Accession Number:
P31213
Ncbi Gene Id Number:
6716
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a microsomal protein expressed at high levels in androgen-sensitive tissues such as the prostate. The encoded protein is active at acidic pH and is sensitive to the 4-azasteroid inhibitor finasteride. Deficiencies in this gene can result in male pseudohermaphroditism, specifically pseudovaginal perineoscrotal hypospadias (PPSH).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

81-624

List Size:
100 uL

List Price:

$540.00

Datasheet
Shipping Info

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$35 Dry Ice Shipment fee may be required on some items.

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