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Antibody used in WB on Human Jurkat 1.25 ug/ml.
Antibody used in CHIP on HCT116 Cells.
Antibody used in WB on Human Jurkat 1.25 ug/ml.

SRF Antibody

CATALOG NUMBER: 31-208

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Dog, Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Dog, Human, Mouse, Rat
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SRF.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
SRF antibody can be used for detection of SRF by ELISA at 1:1562500. SRF antibody can be used for detection of SRF by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1205 - Jurkat Cell Lysate
Predicted Molecular Weight:
52 kDa
Purification:
Antibody is purified by protein A chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store SRF antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
SRF
Additional Names:
SRF, MCM1
Protein Accession Number:
NP_003122
Protein Gi Number:
4507205
Ncbi Gene Id Number:
6722
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
SRF is a ubiquitous nuclear protein that stimulates both cell proliferation and differentiation. It is a member of the MADS (MCM1, Agamous, Deficiens, and SRF) box superfamily of transcription factors. This protein binds to the serum response element (SRE) in the promoter region of target genes. This protein regulates the activity of many immediate-early genes, for example c-fos, and thereby participates in cell cycle regulation, apoptosis, cell growth, and cell differentiation. This gene is the downstream target of many pathways; for example, the mitogen-activated protein kinase pathway (MAPK) that acts through the ternary complex factors (TCFs).
Background Reference 1:
Doi, H., et al., (2005) Arterioscler. Thromb. Vasc. Biol. 25 (11), 2328-2334.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

31-208

List Size:
100 ul

List Price:

$473.00

Datasheet
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