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HEK293 overexpressing Human srGAP2 and probed with 42-786 at 2.5ug/ml in the right panel. Data kindly provided by Ms. Ya-Jing Mi and Dr.Wei-Lin Jin, Institute of Neurosciences, Shanghai Jiao Tong University.
42-786 (0.1ug/ml) staining of Rat Cortical Neuron lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Data kindly provided by Ms. Ya-Jing Mi and Dr.Wei-Lin Jin, Institute of Neurosciences, Shanghai Jiao Ton
HEK293 overexpressing Human srGAP2 and probed with 42-786 at 2.5ug/ml in the right panel. Data kindly provided by Ms. Ya-Jing Mi and Dr.Wei-Lin Jin, Institute of Neurosciences, Shanghai Jiao Tong University.

SRGAP2 Antibody

CATALOG NUMBER: 42-786

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-KQEDRQTPRSPDST
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 124kDa band observed in lysates of cell line HeLa and in rat primary culture cortical neurons at Day 4 (calculated MW of 121kDa according to human NP_056141.2 but the rat protein sequence is stlll provisional). Recommended concentration: 0.1-0.3ug/ml.Immunofluorescence: Transfected HEK293 transiently expressing GFP-tagged Human srGAP2 with the primary antibody stained in the red channel (Confocal IF).
Specificity:
This antibody is expected to recognize isoform a (NP_056141.2).
Positive Control 1:
Cat. No. 1201 - HeLa Cell Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
SRGAP2
Additional Names:
SRGAP2, SLIT-ROBO Rho GTPase activating protein 2, FNBP2, KIAA0456, srGAP3, formin binding protein 2
Protein Accession Number:
NP_056141.2
Protein Gi Number:
112363100
Ncbi Gene Id Number:
23380

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-786

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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