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Western blot analysis of extracts of various cell lines, using SRP72 antibody (13-852) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 90s.
Western blot analysis of extracts of various cell lines, using SRP72 antibody (13-852) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.

SRP72 Antibody

CATALOG NUMBER: 13-852

Clonality:
Polyclonal
Tested Applications:
Flow, IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
A synthetic peptide of human SRP72
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:20 - 1:50
IF: 1:20 - 1:50
Flow: 1:20 - 1:50
Positive Control 1:
HeLa
Positive Control 2:
Jurkat
Positive Control 3:
LO2
Positive Control 4:
U-87MG
Predicted Molecular Weight:
Observed: 75kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
SRP72
Additional Names:
Signal recognition particle subunit SRP72, SRP72, Signal recognition particle 72 kDa protein, SRP72
Ncbi Gene Id Number:
6731
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes the 72 kDa subunit of the signal recognition particle (SRP), a ribonucleoprotein complex that mediates the targeting of secretory proteins to the endoplasmic reticulum (ER). The SRP complex consists of a 7S RNA and 6 protein subunits: SRP9, SRP14, SRP19, SRP54, SRP68, and SRP72, that are bound to the 7S RNA as monomers or heterodimers. SRP has at least 3 distinct functions that can be associated with the protein subunits: signal recognition, translational arrest, and ER membrane targeting by interaction with the docking protein. Mutations in this gene are associated with familial bone marrow failure. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

13-852

List Size:
100 uL

List Price:

$423.00

Datasheet
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