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Western blot analysis of SRPX1 in SK-N-SH cell lysate with SRPX1 antibody at 0.25 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Immunohistochemistry of SRPX1 in human brain tissue with SRPX1 antibody at 5 μg/mL.
Immunofluorescence of SRPX1 in human brain tissue with SRPX1 antibody at 20 μg/mL.
Western blot analysis of SRPX1 in SK-N-SH cell lysate with SRPX1 antibody at 0.25 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Trial Size

SRPX1 Antibody

CATALOG NUMBER: 5473

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
SRPX1 antibody was raised against a 13 amino acid synthetic peptide from near the amino terminus of human SRPX1.
The immunogen is located within amino acids 30 - 80 of SRPX1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
SRPX1 antibody can be used for detection of SRPX1 by Western blot at 0.25 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1220 - SK-N-SH Cell Lysate
Positive Control 2:
Cat. No. 10-301 - Human Brain Tissue Slide
Purification:
SRPX1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
SRPX1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
SRPX1 antibody can be stored at 4°C, stable for up to one year. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SRPX
Additional Names:
SRPX1 Antibody: DRS, ETX1, SRPX1, HEL-S-83p, Sushi repeat-containing protein SRPX
Protein Accession Number:
NP_006298
Protein Gi Number:
5454086
Ncbi Gene Id Number:
8406
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
SRPX1 Antibody: SRPX1 was initially identified as a candidate gene for X-linked retinitis pigmentosa and is overexpressed in the trabecular network of the eye in glaucoma conditions. Its expression is significantly downregulated in a number cancer cell lines and malignant tumor tissues and its ectopic expression induces apoptosis through the endoplasmic reticulum and the activation of caspase-12, -9, and -3, indicating that it can function as a tumor suppressor. SRPX1 is also involved with the regulation of autophagy under low serum conditions and can associate with Rab24, a member of the Rab GTPase protein family that is involved in autophagy. This association of SRPX1 with Rab24 is enhanced during autophagy.
Background Reference 1:
Dry KL, Aldred MA, Edgar AJ, et al. Identification of a novel gene, ETX1 from Xp21.1, a candidate gene for X-linked retinitis pigmentosa (RP3). Hum. Mol. Genet.1995; 4:2347-53.
Background Reference 2:
Iragavarapu S, Algeciras ME, Lee RK, et al. ETX1 is over-expressed in the glaucomatous trabecular meshwork. Mol. Vision2009; 15:2061-7.
Background Reference 3:
Shimakage M, Kawahara K, Kikkawa N, et al. Downregulation of drs mRNA in human colon adenocarcinomas. Int. J. Cancer2000; 87:5-11.
Background Reference 4:
Tambe Y, Isono T, Haraguchi S, et al. A novel apoptotic pathway induced by the drs tumor suppressor gene. Oncogene2004; 23:2977-87.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5473

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

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