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43-680 (0.1ug/ml) staining of Daudi (A) and Mouse Spleen (B) lysates (35ug protein in RIPA buffer). Detected by chemiluminescence.
43-680 (0.1ug/ml) staining of Rat Testis lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
43-680 (5ug/ml) staining of paraffin embedded Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
43-680 (0.1ug/ml) staining of Daudi (A) and Mouse Spleen (B) lysates (35ug protein in RIPA buffer). Detected by chemiluminescence.

SRRT / ARS2 Antibody

CATALOG NUMBER: 43-680

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse, Rat
Homology:
Expected Species Reactivity based on sequence homology: Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-DPRAIVEYRDLDAPD
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 140kDa band observed in lysates of cell line Daudi and of Mouse Spleen, and approx 150kDa band observed in lysates of Rat Testis (calculated MW of 101kDa according to Human NP_056992.4, 100kDa according to Mouse NP_113582.1 and 88.4kDa according to Rat XP_006221478.1). The observed molecular weight is explained by the acetylation of the protein and it corresponds to earlier findings with different antibodies from other commercial sources. An additional band of unknown identity was also consistently observed at 37kDa in Mouse Spleen. This band was successfully blocked by incubation with the immunizing peptide. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Uterus. Recommended concentration: 5ug/ml.
Specificity:
This antibody is expected to recognize all reported isoform (NP_056992.4; NP_001122324.1; NP_001122325.1; NP_001122326.1).
Predicted Molecular Weight:
Approx 140kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
SRRT / ARS2
Additional Names:
SRRT, serrate RNA effector molecule homolog (Arabidopsis), ARS2, ASR2, serrate, arsenate resistance protein 2, arsenate resistance protein ARS2, arsenite resistance protein, arsenite-resistance protein 2, serrate RNA effector molecule homolog
Protein Accession Number:
NP_056992.4,NP_001122324.1,NP_001122325.1,NP_001122326.1
Protein Gi Number:
58331218
Ncbi Gene Id Number:
51593
Background Reference 1:
Gruber JJ, Olejniczak SH, Yong J, La Rocca G, Dreyfuss G, Thompson CB.Ars2 promotes proper replication-dependent histone mRNA 3' end formation.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-680

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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