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Western blot analysis of extracts of various cell lines, using SSBP1 antibody (22-609) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunohistochemistry of paraffin-embedded human colon carcinoma using SSBP1 antibody (22-609) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast cancer using SSBP1 antibody (22-609) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spinal cord using SSBP1 antibody (22-609) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using SSBP1 antibody (22-609) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using SSBP1 antibody (22-609). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of MCF7 cells using 1ug SSBP1 antibody (22-609). Western blot was performed from the immunoprecipitate using SSBP1 antibody (22-609) at a dilition of 1:1000.
Western blot analysis of extracts of various cell lines, using SSBP1 antibody (22-609) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.

SSBP1 Antibody

CATALOG NUMBER: 22-609

Clonality:
Polyclonal
Tested Applications:
IF, IHC, IP, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-148 of human SSBP1 (NP_001243442.1).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, IP, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:100
IP: 1:50 - 1:200
Positive Control 1:
HL-60
Positive Control 2:
MCF7
Positive Control 3:
SW620
Predicted Molecular Weight:
Observed: 14kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
SSBP1
Additional Names:
Mt-SSB, SOSS-B1, SSBP, mtSSB, single-stranded DNA-binding protein, mitochondrial, PWP1-interacting protein 17, single-stranded DNA binding protein 1, mitochondrial
Ncbi Gene Id Number:
6742
User Note:
Optimal dilutions for each application to be determined by the researcher.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

22-609

List Size:
100 uL

List Price:

$436.00

Out of stock

Datasheet
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