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Western blot analysis of extracts of various cell lines, using SSC4D antibody (16-344) at 1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
Western blot analysis of extracts of various cell lines, using SSC4D antibody (16-344) at 1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.

SSC4D Antibody

CATALOG NUMBER: 16-344

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 446-575 of human SSC4D (NP_542782.1).
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
WB: 1:500 - 1:2000
Positive Control 1:
U-87MG
Positive Control 2:
HepG2
Positive Control 3:
Mouse liver
Positive Control 4:
Mouse brain
Positive Control 5:
Rat liver
Positive Control 6:
Rat brain
Predicted Molecular Weight:
Observed: 61kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
SSC4D
Additional Names:
Scavenger receptor cysteine-rich domain-containing group B protein {ECO:0000303, PubMed:12466895, ECO:0000312, HGNC:HGNC:14461}, Four scavenger receptor cysteine-rich domains-containing protein {ECO:0000312, HGNC:HGNC:14461}, S4D-SRCRB, SSC4D (HGNC:14461)
Ncbi Gene Id Number:
136853
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The scavenger receptor cysteine-rich (SRCR) superfamily is an ancient and highly conserved group of cell surface and/or secreted proteins, some of which are involved in the development of the immune system and the regulation of both innate and adaptive immune responses. Group B SRCR domains usually contain 8 regularly spaced cysteines that give rise to a well-defined intradomain disulfide-bond pattern.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

16-344

List Size:
100 uL

List Price:

$436.00

Datasheet
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