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Western Blot at 1:1000 dilution + ZR-75-1 whole cell lysate Lysates/proteins at 20 ug per lane.
SSH3 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Confocal immunofluorescent analysis of SSH3 Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
Western Blot at 1:1000 dilution + ZR-75-1 whole cell lysate Lysates/proteins at 20 ug per lane.

SSH3 Antibody

CATALOG NUMBER: 55-994

Clonality:
Polyclonal
Tested Applications:
Flow, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This SSH3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 575-602 amino acids from the C-terminal region of human SSH3.
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
For IF starting dilution is: 1:10~50
Predicted Molecular Weight:
73 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SSH3
Additional Names:
Protein phosphatase Slingshot homolog 3, SSH-like protein 3, SSH-3L, hSSH-3L, SSH3, SSH3L
Protein Accession Number:
Q8TE77
Protein Gi Number:
82582268
Ncbi Gene Id Number:
54961
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The ADF (actin-depolymerizing factor)/cofilin family (seeMIM 601442) is composed of stimulus-responsive mediators of actindynamics. ADF/cofilin proteins are inactivated by kinases such asLIM domain kinase-1 (LIMK1; MIM 601329). The SSH family appears toplay a role in actin dynamics by reactivating ADF/cofilin proteinsin vivo (Niwa et al., 2002 [PubMed 11832213]).
Background Reference 1:
Kousaka, K., et al. Genesis 46(5):246-255(2008)
Background Reference 2:
Kligys, K., et al. J. Biol. Chem. 282(44):32520-32528(2007)
Background Reference 3:
Beausoleil, S.A., et al. Nat. Biotechnol. 24(10):1285-1292(2006)
Background Reference 4:
Soosairajah, J., et al. EMBO J. 24(3):473-486(2005)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-994

List Size:
400 ul

List Price:

$495.00

Datasheet
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